| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 臺灣大學 | 吳如雄,林上智,謝侑靜,毛文玲 | 04:09.64 | 50M: 27.69, 100M: 57.64, <->M: + 0.38, 150M: 1:24.43, 200M: 1:53.94, <->M: + 0.54, 250M: 2:26.33, 300M: 3:02.84, <->M: + 0.60, 350M: 3:33.50, 400M: 4:09.64 | q01 | |
| 臺灣體大 | 金著恩,黃唯鑫,侯又嘉,葉璟雯 | 04:09.66 | 50M: 28.38, 100M: 59.42, <->M: + 0.53, 150M: 1:28.89, 200M: 1:59.93, <->M: + 0.59, 250M: 2:31.00, 300M: 3:05.06, <->M: + 0.66, 350M: 3:36.51, 400M: 4:09.66 | q02 | |
| 東吳大學 | 黃 翊,黃心宇,林沅臻,葉晨霖 | 04:10.17 | 50M: 27.34, 100M: 57.09, <->M: + 0.25, 150M: 1:24.69, 200M: 1:56.94, <->M: + 0.48, 250M: 2:27.57, 300M: 3:02.48, <->M: + 0.55, 350M: 3:34.13, 400M: 4:10.17 | q03 | |
| 高雄科大 | 張晏鑫,張倚瑄,邱于綸,王哲明 | 04:13.56 | 50M: 28.08, 100M: 59.65, <->M: + 0.17, 150M: 1:33.03, 200M: 2:09.88, <->M: + 0.11, 250M: 2:41.41, 300M: 3:15.88, <->M: + 0.16, 350M: 3:43.21, 400M: 4:13.56 | q04 | |
| 中央大學 | 楊方硯,孫秉蔚,洪琬哲,郭郁炩 | 04:14.00 | 50M: 28.60, 100M: 58.78, <->M: + 0.56, 150M: 1:25.67, 200M: 1:54.75, <->M: + 0.50, 250M: 2:27.72, 300M: 3:06.61, <->M: + 0.38, 350M: 3:38.14, 400M: 4:14.00 | q05 | |
| 成功大學 | 黃柏睿,劉 樺,黃甜兒,賴昱豪 | 04:16.07 | 50M: 27.06, 100M: 59.22, <->M: + 0.57, 150M: 1:32.05, 200M: 2:10.31, <->M: + 0.47, 250M: 2:41.78, 300M: 3:17.73, <->M: + 0.40, 350M: 3:45.37, 400M: 4:16.07 | q06 | |
| 政治大學 | 何家翔,毛怡蓁,徐嘉妤,吳岱融 | 04:17.21 | 50M: 29.31, 100M: 1:02.50, <->M: + 0.48, 150M: 1:35.45, 200M: 2:12.47, <->M: + 0.40, 250M: 2:44.03, 300M: 3:19.69, <->M: + 0.44, 350M: 3:46.90, 400M: 4:17.21 | q07 | |
| 陸軍官校 | 朱智陽,林 翎,莊佳軒,蘇哲賢 | 04:19.10 | 50M: 29.64, 100M: 1:02.84, <->M: + 0.35, 150M: 1:33.91, 200M: 2:08.04, <->M: + 0.58, 250M: 2:38.96, 300M: 3:16.08, <->M: + 0.60, 350M: 3:46.84, 400M: 4:19.10 | q08 | |
| 清華大學 | 石柏昱,羅文韻,林子云,王湘凱 | 04:25.84 | 50M: 28.91, 100M: 1:00.24, <->M: + 0.52, 150M: 1:33.35, 200M: 2:12.49, <->M: + 0.46, 250M: 2:46.16, 300M: 3:24.84, <->M: + 0.63, 350M: 3:54.30, 400M: 4:25.84 | | |
| 輔仁大學 | 陳琮尹,陳采渝,吳珮慈,溫柏翔 | 04:27.29 | 50M: 28.15, 100M: 58.00, <->M: + 0.47, 150M: 1:33.88, 200M: 2:12.57, <->M: + 0.54, 250M: 2:47.16, 300M: 3:28.10, <->M: + 0.40, 350M: 3:57.25, 400M: 4:27.29 | | |
| 東華大學 | 王維安,曾柏銘,曾柏瑜,藍苡瑄 | 04:29.19 | 50M: 29.05, 100M: 1:02.23, <->M: + 0.38, 150M: 1:31.39, 200M: 2:03.67, <->M: + 0.51, 250M: 2:39.14, 300M: 3:17.96, <->M: + 0.36, 350M: 3:50.97, 400M: 4:29.19 | | |
| 臺北科大 | 李宸宇,劉予安,張茹庭,陳彥至 | | 50M: 29.81, 100M: 1:02.91, <->M: + 0.24, 150M: 1:39.28, 200M: 2:25.58, <->M: + 0.69, 250M: 3:01.34, 300M: 3:41.23, <->M: + 0.36, 350M: 4:09.23, | | 犯規 |
| 國防醫學院 | 鄭尚昀,劉仲慈,李品儀,廖子權 | | 50M: 28.78, 100M: 1:01.34, <->M: + 0.55, 150M: 1:35.46, 200M: 2:21.26, <->M: + 0.43, 250M: 2:53.41, 300M: 3:29.05, <->M: + 0.60, 350M: 4:01.54, | | 逾時 |
| 陽明交大 | 杜彥呈,賴楙勛,千年倫子,林家伊 | | 50M: 29.24, 100M: 1:00.37, <->M: + 0.71, 150M: 1:29.87, 200M: 2:02.11, <->M: + 0.48, 250M: 2:39.21, 300M: 3:21.49, <->M: + 0.51, 350M: 3:56.80, | | 逾時 |
| 東海大學 | 陳冠妤,蔡宜宸,陳孝齊,陳彥霖 | | 50M: 38.35, 100M: 1:25.46, <->M: + 0.69, 150M: 2:13.52, 200M: 2:40.61, <->M: + 0.59, 250M: 3:53.49, , , , | | 逾時 |
| 中山醫大 | 周依璇,張皓翔,丁郁倩,宋 垣 | | 50M: 34.87, 100M: 1:14.16, <->M: + 0.78, 150M: 1:44.17, 200M: 2:19.12, <->M: + 0.75, 250M: 2:56.85, 300M: 3:43.83, , 350M: 4:21.23, | | 逾時 |
| 淡江大學 | 孔令禹,黃琪淇,黃心垚,譚栢熙 | | 50M: 29.32, 100M: 1:01.01, <->M: + 0.34, 150M: 1:36.32, 200M: 2:15.51, <->M: + 0.51, 250M: 2:50.27, 300M: 3:31.68, <->M: + 0.13, 350M: 4:00.12, | | 逾時 |
| 臺北醫大 | 葉蕙瑜,王豫延,朱村恩,賴宥安 | | 50M: 35.07, , , 150M: 1:52.96, 200M: 2:28.15, <->M: + 0.38, 250M: 3:04.34, 300M: 3:46.64, <->M: + 0.51, 350M: 4:14.56, | | 逾時 |
| 臺灣師大 | | | | | 請假 |